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Image Search Results
Journal: Stem Cell Reports
Article Title: Protein-encapsulated doxorubicin reduces cardiotoxicity in hiPSC-cardiomyocytes and cardiac spheroids while maintaining anticancer efficacy
doi: 10.1016/j.stemcr.2023.08.005
Figure Lengend Snippet: SPEDOX-6 induces less functional toxicity than UF DOX in hiPSC-CMs (A) hiPSC-CM beat rate normalized to DMSO after 3 days of UF DOX or SPEDOX-6 treatment. See . ∗ p < 0.05 between DMSO and other groups, determined by one-way ANOVA with Tukey’s post hoc test. n = 4 independent experiments. (B) Representative field potential recordings from contracting hiPSC-CMs in multielectrode arrays treated with DMSO, UF DOX, or SPEDOX-6 for up to 72 h. (C) Average spike amplitude mean and field potential duration (FPD) mean from field potential recordings of contracting hiPSC-CMs in multielectrode arrays treated with DMSO, UF DOX, or SPEDOX-6 for up to 72 h, corresponding with (B). n = 9 technical replicates. Error bars represent SD. (D) Live fluorescence imaging of ACTN2-GFP hiPSC-CMs subjected to DMSO, UF DOX, or SPEDOX-6 for up to 72 h. α-actinin (green) represents a cardiomyocyte-specific protein marking the striated cardiac sarcomeres in live hiPSC-CMs. DOX (red) shows DOX intracellular accumulation. (E) SarcTrack dataset showing representative sarcomere displacement timegraphs of ACTN2-GFP hiPSC-CMs treated with DMSO, UF DOX, or SPEDOX-6 for 72 h. See . (F) SarcTrack-based quantification of sarcomere displacement during the hiPSC-CM contraction cycle in ACTN2-GFP hiPSC-CMs treated with DMSO, UF DOX, or SPEDOX-6 for 72 h. See . n = 10, 29, and 22 sarcomeres were detected for DMSO, UF DOX, and SPEDOX-6 conditions, respectively. (G) Calcium imaging timegraphs of WTC-GCaMP hiPSC-CMs treated with DMSO, UF DOX, or SPEDOX-6 for 72 h. ΔF/F0 compares the change of the fluorescence intensity to the baseline fluorescence intensity before the contraction. See .
Article Snippet: Videos were analyzed in
Techniques: Functional Assay, Fluorescence, Imaging